李智立

公卫考场

研究方向

本课题组主要采用生物分析化学和质谱技术(FT ICR MS)，与临床医学紧密结合： 
(1) 开展与肿瘤早期诊断、治疗和预后判断相关的研究工作：肿瘤相关生物标志物筛选；肿瘤个体化治疗和转化医学研究；血清/血浆成分分析新技术和新方法研究及其应用；生物大分子复合物(体)结构、功能与肿瘤病变关系研究.糖蛋白糖链结构分析方法研究及其应用。  公卫百科
(2) 生物分子研究新技术和新方法：质谱分子成像技术、新型生物分析化学方法的开发。

主要学习及工作简历 公卫考场

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社会任职 公卫百科

中国生物物理学会常务理事，北京市理化分析测试技术学会副理事长

公卫百科

代表性学术论文 公卫家园

1. Guoqiang Chen, Hui Liu, Xiaodong Wang, Zhili Li*, In vitro methylation by methanol: Proteomic screening and prevalence investigation, Analytica Chimica Acta, 2010, 661，76-75 (IF:3.14)

2. Zhiili Li, Feng Song, Zhihao Zhuang, Debra Dunaway-Mariano and Karen S. Anderson*, Monitoring Enzyme Catalysis in the Multimeric State: Direct Observation of Arthrobacter 4-Hydroxybenzoyl-Coenzyme A Thioesterase Catalytic Complexes using Time-resolved Electrospray Ionization Mass Spectrometry. Analytical Biochemistry, 2009, 394, 209-216 (IF:3.088). 公卫论坛

3. Chen Guo-Qiang, Liu Hui, Zhang Hai-Jing, Deng Yan-Chun, Li Zhi-Li*, Proteomic Characterization of Human Erythrocyte 20S Proteasome and Analysis of Species-dependent 20S Proteasome Heterogeneity, Chinese Journal of Analytical Chemistry, 2009, 37(12), 1711–1716.(IF: 0.633 ) 公卫考场

4. Guoqiang Chen, Yuanming Luo, Xinli Wang, Zhiyun Zhao, Hui Liu, Haijing Zhang, Zhili Li*, A relatively simple and economical protocol for proteomic analyses of human 20S proteasome: compatible with both scaled-up and scaled-down purifications, Electrophoresis, 2009, 30, 2422-2430. (IF:3.509) 公卫论坛

5. Haijing Zhang, Liyan Lv, Hui Liu, Lei Cui, Guoqiang Chen, Peixuan Bi, Zhili Li*, Profiling the potential biomarkers for cell differentiation of pancreatic cancer using iTRAQ and 2-D LC-MS/MS, Proteomics-Clinical Application, 2009, 3, 862-871. (IF:1.500) 公卫家园

6. Zhili Li* and Apurba Kumar Sau, Structural studies on Helicobacter pylori 3-Deoxy-D-manno-2-octulosonate-8-phosphate synthase using electrospray ionization mass spectrometry：a tetrameric complex composed of dimeric dimers, Rapid Communications in Mass Spectrometry, 2009, 23(11), 1573-1578. (IF:2.98) 公卫百科

7. Zhili Li* Apurba K. Sau, Probing the subunit-subunit interaction of the tetramer of E. coli KDO8P synthase by electrospray ionization mass spectrometry, Chinese Journal of Chemistry, 2009,27,111-1116.( IF: 0.945)

公卫考场

8. Li Zhi-li*, Effects of temperature and energy on stability of oligomeric enzyme probed on electrospray ionization mass spectrometry, Chemical Research in Chinese Universities, 2008, 24(6), 771-777.( IF:0.408)

9. Zhili Li, Apurba K. Sau, Cristina M. Furdui and Karen S. Anderson*, Probing the role of tightly bound phosphoenolpyruvate in Escherichia coli 3-deoxy-d-manno-octulosonate 8-phosphate synthase catalysis using quantitative time-resolved electrospray ionization mass spectrometry in the millisecond time range, Analytical Biochemistry, 2005, 343(1), 35-47. (IF:3.08) 公卫家园

10. Apurba K. Sau, Zhili Li and Karen S Anderson, Probing the role of metal ions in the catalysis of Helicobacter pylori 3-deoxy-D-manno-octulosonate-8-phosphate synthase using a transient kinetic analysis, The Journal of Biological Chemistry, 2004, 279(16), 15787-15794. (IF: 6.8)

11. Zhili Li, Apurba Sau, Shida Shen, Craig Whitehouse, Timor Baasov and Karen S. Anderson* , A snapshot of enzyme catalysis using electrospray ionization mass spectrometry, Journal of the American Chemical Society，2003,125, 9938-9939. (IF:7.9) 公卫考场

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联系方式 公卫论坛

     邮箱：lizhili@ibms.pumc.edu.cn

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